David Mai (he/him) is a postdoc at the Fred Hutch Cancer Center. Previously, he completed his PhD in Bioengineering at the University of Pennsylvania, where he worked on engineering therapeutic T cells for solid tumor applications. In the Srivatsan lab, David is exploring genomic technology development and its applications in cell engineering and protein design.
This study presents a novel semi-permeable capsule (SPC) that isolates single cells while allowing selective biomolecular exchange, retaining larger biomolecules like genomic DNA while permitting smaller molecules to pass through. Using SPC-based single-cell whole genome sequencing (sc-WGS), researchers achieved high sensitivity and specificity in detecting genomic variants and successfully reconstructed cellular genealogies from cells harboring the hypermutator polymerase ε allele (POLE P286R).
