Sam Sgrizzi (she/her) is a graduate student in the Molecular and Cellular Biology program at the University of Washington and Fred Hutch. As an undergrad at Boston University, Sam conducted biochemical research at BU’s Cytoskeleton and Signaling Lab, but later became interested in genome engineering and biotechnology. She spent a year at Boston-based startup Accure Health, helping design nano vesicle-based biomarker assays. Afterwards, Sam joined the Abudayyah-Gootenberg Lab at Harvard Medical School, where she developed programmable tools for gene editing and RNA sensing. In the Srivatsan Lab, Sam is now exploring microfluidics-based gene synthesis and single-cell genomics.
This study presents a novel semi-permeable capsule (SPC) that isolates single cells while allowing selective biomolecular exchange, retaining larger biomolecules like genomic DNA while permitting smaller molecules to pass through. Using SPC-based single-cell whole genome sequencing (sc-WGS), researchers achieved high sensitivity and specificity in detecting genomic variants and successfully reconstructed cellular genealogies from cells harboring the hypermutator polymerase ε allele (POLE P286R).
